Office of laboratory and workshop affairs

One the important and active departments of the deputy of research and technology is the office of laboratory and workshop affairs which has a great role in Islamic Azad University, Kermanshah Branch (henceforth IAUKSH) ranking as well as the development of research and education section as well as research projects.

It is worth mentioning that five laboratories are equipped with high tech facilities such as GC, HPLC, FTIR, spectrometers, potentiostat, galvanostat, polarography and the like. The establishment of the a genomics and diametric laboratory is a unique facility in the west of Iran, which thanks to its expert personnel has made it possible for our-of-university demands for upon-request analysis to be carried out after appropriate permissions are obtain from its related technicians.

The office of laboratories and workshops of Islamic Azad University, Kermanshah Branch at a glance

It is an important and active department of the deputy of research and technology of the university and plays substantial role in contributing to the development of research and education section as well as research projects.

The office of laboratories of IAUKSH were established in 1380 under the management of Mohammad Saeid Veghar. It was initially equipped with 18 laboratories and workshops and then between 1383 and 1388 with the management of Mohammad Hadi Haqiqi this number grew to 70 laboratories and workshops. Finally in Changiz Karami’s term, from 1388 to 1390, the number of laboratories and workshops rose to 110.

Currently Mr Mahdi Emdadi is the manager of laboratories at IAUKSH.

Some the responsibilities of the manager of Laboratories includes the following:

  • Supervising laboratory and research related equipment purchases
  • Organizing and budgeting the use of laboratory spaces
  • Synchronizing student researchers and instructors so that they can make a better use of the laboratory spaces, equipment, and facilities.
  • Quality checking the equipment and facilities of the laboratories against the standards
  • Establishing and/or equipping any new lab or workshops, which plays a substantial role in raising the IAUKSH ranking

It is worth mentioning that five laboratories are equipped with high tech facilities such as GC, HPLC, FTIR, spectrometers, potentiostat, galvanostat, polarography and the like. The establishment of the a genomics and diametric laboratory is a unique facility in the west of Iran, which thanks to its expert personnel has made it possible for our-of-university demands for upon-request analysis to be carried out after appropriate permissions are obtain from its related technicians.

Agriculture research laboratory

Agriculture research laboratory was establish by Dr. Mohammad Jowkar a faculty member of the department of Agriculture and plant breeding by an order from the deputy of research in an area with approximately 100 square meters on the campus of Imam Khomeini (PBUH) complex in 25/8/1388. Later in Khordad 1389 the lab was inaugurated by Dr. Jasebi.

Goals

The purpose for the establishment of the lab is to prepare the grounds for the development and progression of creativity and research at university. It is done so through esteeming research and researchers besides avoiding complexities researches might face when some of the classes were held in labs. Therefore the labs are able to offer their services to all faculty members, university personnel, and students from other universities.

Equipment

In addition to offering such equipment as scales with the precision of one ten thousandth, Ph meter, auto-color, incubator, chemical hood, various heaters and stirrers, this lab provides the following facilities

  1. High Performance Liquid Chromatography (HPLC)
  2. All the equipment needed for physiological studies such as sun-scan, porometer (measuring the area of the stomata openings of a leaf by the amount of a gas passing through a given area of it), fluorometer (measuring the intensity of fluorescence), chlorometer (measuring the amount of chlorophyll), leaf level meter, stress gauge, an instrument measuring plant water potential.
  3. All equipment needed for culturing plant cell and tissue such as Lab hood, growth chamber, autoclave, shaker, etc.

 

Research projects carried out in the lab

From among the most important research activities conducted by the faculty members and students, one could mention “optimizing the protocol for cario-typing of Narcissus, optimizing the protocol for Endive/chicory tissue culture, optimizing the protocol for endive/chicory cell culture as well as the physiological studies of after harvest of ornamental plants such as Alstroemeria, Mary, and Rose.

The ongoing research projects

Investigating the effects of some combinations on microbial contamination of vase solution of Alstroemeria cut flower.

M.A. theses conducted in these labs include the following

  1. A comparison of two methods for germplasm conservation of cichorium intybus l. by cryopreservation of callus and shoot tip and evaluation of genetic stability in cryopreserved stocks.
  2. Cryopreservation of  Cichorium Intybus  L. seeds by vitrification through

 

On-going M.A. theses in these labs include the following

  1. Determining
  2. Analyzing the effect of
  3. Evaluating
  4. Evaluating
  5. evaluaitng

 

Research projects conducted or ongoing in young researchers’ club

  1. Determining the need
  2. Studying the effect

Research projects conducted or ongoing in these laboratories

  1. Analyzing the
  2.  

M.A level labs of Agriculture department (plant breeding)

M.A. level labs of agriculture department (M.A. in plant breeding) were established (by Ms Parvin Manuchehri) in an area of 1200 square meters in 1/5/1385. Under such names as genomics, plant tissue culture, and cytogenetic, it has accommodate practical credits offered for a 15 student strong group in an area of 60 square meters.

Upon the establishment of the faculty of sciences in 1/12/1387, this lab was moved to a new building and after proteomics lab was set up, M.A. level labs of agriculture department developed its capacity by opening up two new labs in an area of 90 and 70 square meters each taking 15 students. Because of the rise in the number of M.A. students, these two recent labs under such names as genomics, plant tissue culture, cytogenetic, proteomics and cytology are designated as the place where M.A. level classes are held. Moreover, these labs give laboratory services to IAUKSH students as well as those from the neighboring branches of the university.

 Goals

These labs were established to create an appropriate education-research environment for such plactical classes as cytogenetic, the application of biotechnology in plant breeding, cytology and plant tissue culture for M.A. level students, as well as an environment where these students can conduct research projects or carry out their theses.

Facilities

In this lab besides the conventional lab facilities such as Ph meter, Avon, typical and refrigerator incubators, autoclaves, chemical and laminar lab hoods, shakers, vertexes, lab digital scales, and the like, equipment needed for extraction and analysis of various plant DNAs, various plant tissue culture, as well as extraction and analysis of plant proteins are available.

A.Genomics Laboratory

Equipment installed in this lab

1.Refrigerator centrifuge

Brand name: Hettich

Model: Mikro 200R

It can rotate at 15000 rpm and adjust the temperature at 10 C to separate different phases of the material used.

2.Agagel Mini Electrolysis

Brand name:Biometra

This apparatus is used to make 8 cm thick gels and to separate DNA on them.

3.Agagel Maxi Electrolysis

Brand name:Biometra

This is used to produce 18 cm thick gels

4.Gel documentation

Brand name: Vilber

Model: E-Box vx2

It is equipped with a 104 Megapixel camera and the E-CAPT software to analyze images. It is used to take photos of the gels and their bands by using UV ray.

5.PCR

Brand name: Bioard

Model: I cycler

This is used to set specific temperature profiles as well as specific programs to replicate the DNAs extracted.

B.Proteomics laboratory

The equipment installed in this lab includes the following:

1.IPG Phor

Brand name: GE Healthcare

Model: Ettan IPG phor 3

This is used to separate the first dimension of a 2 dimensional electrolysis. It separate the proteins from each other based on their PH and isoelectric points.

2.Vertical electrolysis

Brand name: Amersham Bioscience

Model: SE600 Ruby

This is used to separate proteins and the second dimension of 2 dimensional electrolysis.

3. Spectrophotometer

Brand name: Nano Vue plus

With this apparatus one can measure the amount of light passing through or being absorbed on different wave lengths, RNA and DNA concentration, proteins. Moreover this apparatus requires a small amount of the sample (about 2 micrometers). It can draw the standard deviation curve and calculate the final concentration of the material.

4.Image scanner III

It is used to can protein gel images and transferring them to a computer. It uses Image Master and can scan two gels simultaneously.

C.Analytical chemistry laboratory

This lab was established by Ms Elaheh Ahmadi (M.Sc. holder in Analytical Chemistry) in the central building of the laboratories in 1387 and was inaugurated by the IAU Higher presidency, Dr Jasbi in Khord 1389.

Analytical chemistry lab as one of the highly equipped labs in IAUKSH, within the university framework provides for part of the research and development needs of industry and university, in particular in particular it produces medical sensors, sensors sensitive to vitamins, hormones and metals, synthesis of mineral nanoparticles and complexes.

It is obvious that economic and social development is bound with development in production and industry. Right now because of the decrease in current earnings and the economic problems our country is grappling with, it behooves the public to decrease our research and industrial dependence on currency. Therefore, chemistry laboratory with its expert personnel and high end equipment is ready to cooperate with industries such as medicine, food, oil, petro-chemistry and related industries to give scientific and research services, specific productions, to carry out research projects.

Equipment

In this lab besides the conventional lab facilities such as scales measuring one ten thousandth of gram, various heater-stirrers, Ph meter, Avon, centrifuges, shakers, microwaves, chemical hoods, water distillers, and the like, is equipped technical potentiostat and galvanostat, polarograph, spectrophotometer, conductometer and Ion meter.

1.Potentiostat and galvanostat (AUTOLAB/PGSSTAT302N)

This high end, high current potentiostat/galvanostat, with a compliance voltage of 30 V and a bandwidth of 1 MHz, combined with our FRA32M module, is specially designed for electrochemical impedance spectroscopy. It has both digital and analogue outputs which enables it to control other apparatuses. With a minor correction or improvement in the apparatus, the user is able to dynamically measure iR-compensation.

2.Polarograph/Metrohm 797

The i797 is used for manual measurements or educational purposes. This system has a high sensitivity when used for voltammeter methods. 

Anything capable of being measured with a Volta-metric method can also be measured with this apparatus. It includes model i797 along with its standard accessories. After connecting to a computer, selecting specific user and retrieving the method from memory, one can start the experiment. It gets connected to a computer through a USB port. It is utilized manually and is used for measuring quantity on a daily basis or for research purposes.

Polarograph/Metrohm is a simple, inexpensive apparatus to measure the quantity of the material such that it traces the measurement of heavy metals some Ions and some organic materials.

3.Spectrophotometer cary100

The samples used with this instrument are readily made and it measures all samples with high precision. The analysis results are of high quality. It uses both the ultraviolet and visible beam of light. Its instructions are monitored by a chip/CPU. The analysis can be repeated with both high precision and sensitivity. The instrument is controlled by a computer and its related software program.